After game is reset something happens

After we lose the game we are then put back to the main menu now the problem is when this happens and we press play again some of our triggers no longer work like our death trigger that kills you instantly. you can walk accross it and the animation plays but no death

Does anyone have any ideas?

The simplest way to return to the main menu is:

Application.LoadLevel(0);

This reloads everything, except global (class) variables.