Are Variables Pre-calculated?

So I’m making a 2D game where everything is aligned with a pixel grid (every pixel is 1/16 unit). Because of this I use numbers like:
float width = 5/16f;
Do variables like these get pre-calculated when compiling? I’m a bit worried about potential performance problems.

A Calculation like that wouldn’t be an issue.

From my experience what lags a system is low memory issues (using high-res graphics/textures) and excessive draw calls (rendering objects to screen). And if you are updating every frame, then you’d have a problem.

But again, doing simple division of even a couple hundred variables wouldn’t do anything to your overall performance on startup, or even during run-time.