Changes not reflected in play mode

In edit mode, I moved around objects and in the Game tab I can see that the objects are in their correct positions, yet when I enter play mode, everything reverts back to where they were. Anyone know the cause?

From Bunny83:

You might have scripts or animations on some objects that cause a translation / rotation of the object. A common mistake are animations that animate the root object. This will usually move the object to 0,0,0