Hi everyone!
I have made several simple games… I use Chartboost for my ads… I just wanna ask when is the best time(effective) to show the ad? on Start? on GameOver? or after the Replay button is tapped?
And how often do you usually show the ad? after every GameOver? or after (int),let’s say 3 gameovers?
My games right now are having ads once they replay and the ad shows every gameover.
I need the best settings not to annoy my users

(some are annoyed with ads while some are not)
Thank you!
I’m using a timer. I’m showing the ad when the player clicks on “Play/Replay” buttons, but only if x minutes have passed since the last ad.
Also, show an ad when the player is trying to exit the game. You can display a “Do you want to exit the game? Yes (or) No” and add an advertisement on top of the exit menu.
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okay…ive made a counter saved on playerprefs and increments after every game… and if it is equal to (int) lets say 5, the ad will show… and i show the ad just after the player dies… 
I have a timer for my interstitial… when the player dies and there has been 5 minutes since the last ad, then i will show it.
I think it’s usually safe to show ads when the user pauses the game, or exits the app.
The right answer is to do A/B testing on the combinations. For example add a switch which is randomly chosen on startup (or better yet a value stored on a server somewhere) to be either Start or GameOver.
Show ads either at the Start or GameOver screen depending on the switch and ensure your analytics events capture when the ad is shown (for example you could track: ShowAdStart, ShowAdGameOver, ClickAdStart, ClickAdGameOver).
See which ads get clicked more often.
You probably also want to measure how the flag effects retention. For example at GameAnalytics.com you could set up a funnel to see how many users who are shown ads at start go on to reach level 5, level 10, level 20, etc. Compare the drop off points to those shown ads at GameOver.
I use banners in menu, and pause menu, and I use interstitial on gameover. But I like the timer idea instead of every game over screen, I’ll definitely do that. Might be less frequent popups but makes your user wanna switch off the internet less, and avoid ads all together, I guess. xD