Force refresh of frame

How I can to refrsh frame. I try to shot screenshot with some change before.

but the frame not updating before screenshot.

Camera.Render

not working for me, im trying to place a sprite twice with only one gameObject, i tried to force the camera to render when the sprite is on a diferent position than actual, and the camera with Don´t Clear, to display all of it, and that don´t work

my game needs to have that, because i use a several amount of GameObjects, just for render a Tilemap with ilumination

Today, my game creates and destroy the tiles than i don´t use because is not on camera, but, still with lag cuz of the several amount of GameObjects.

So I need to display all sprite renderers with all the materials without have those amounts of gameObjects