Is instantiating prefabs on awake more expensive to performance than setting prefabs in the scene view?

I have a script that instantiates many trigger prefabs in designated spots on awake. Does this have more of a performance impact than simply setting the prefabs in unity’s scene view editor (not in-game)? Or does it really not make a difference? Thanks for reading.

Instantiating Prefab is costly then referencing the gameobject and the enabling and disabling them when needed