My Canvas changed UI scale in all scenes

I’ve changed layouts in the Unity interface and for some reason the Canvas UI elements have all halved in size, in all my scenes. Nothing I can fix with the Scale slider above the Game view, nor with the Canvas Scaler (it is scene-specific). Any idea what could have happened here? I’m sure this can be fixed somehow instead of having to resize all my UI elements to correct values (the values haven’t changed). Thanks.

I can’t remember, but does changing layout also reset the resolution of your game window? If your game window resolution has changed I think it could mess with the UI depending on how they are anchored.