Red line on the middle of game screen

There is a red/yellowish line in the middle of my Game screen, on the Tilemap it looks perfectly fine, it only appears once I “run” the game. I have Anti Aliasing disabled and Filter Mode set to “point (no filter)” on my sprites. It doesn’t matter which sprites I use, the line remains the same.
I don’t know how to fix this issue. I’ve watched several videos about how to fix “tearing” or “seams” but nothing has helped. If anyone could point me in the right direction I would be extremely grateful.

6 months later and I’m having the same issue. Also can’t find a solution elsewhere online. Can someone please answer this topic? Cheers in advance.

So, update for anyone else who finds this issue; Out of frustration, I was also reading through notes on the Zenva Academy lesson I’m taking where I encountered this issue; Someone added a reply that, while in reference to a different visual pixel issue, solved this one, as well as my own subsequent testing with it. #1 worked for me and was the far simpler solution; But if for whatever reason that doesn’t work, or you want to solve this issue for all resolutions, you can try #2.

  1. Try adjusting your “Game” resolution. I had this issue on 16:9, but as soon as I set it to 1920x1080, on top of looking better, all of these lines (That would previously change position based on my camera movement in 16:9) stopped appearing.

  2. A suggestion on the Zenva Academy course notes: “Make the sprite’s pixels per unit value slightly smaller (around 0.5). This will make the sprites very slightly overlap (not really noticable at all) and will fix the lines.”

Hope this helps anyone else scouring the internet for answers/help with this same problem. Cheers.