Resources drawbacks

Is there any drawback to putting my game’s assets into a Resources folder?

I understand the impact on a game designed for the web, but what about windows and mac player games?

Is there any benefit to not using a Resources folder, or being very frugal about what elements can be loaded dynamically?

With a standalone build, anything inside the Resources folder will be added to the build, whether it’s used or not. So it’s good practice to only put in there what you need, or every build you do will be come bloated, and require more and more time to build.

That holds for any build.
The Resources folder is for dynamically created objects and has to be always present in the project as there isn’t a godlike method to know which objects the scripts are going to request from the resources.