scene window sizing to match game resolution ?

Im making a game that is 800 x 600 resolution but when i set things up in the scene editor it looks good, but when i build and run items arent where they are supposed to be - if i were to make my scene window 800 x 600 would my "in game view" be accurate to when i build and run ?

Thanks in advance :)

edit

i have a gui label with my game title as a texture. instead of "dots" covering the i's i want animated fire sprites. i seem to have to hand place the sprites in order to get it to work , but when i place them in the scene and hit the "play" button it looks good but when i "build and run" the sprites are out of place.

i basically want to hit the "play" button and have it be my 800x600 resolution so i can move sprites around accurately instead of having to "build and run" every 2 seconds to see if i got things where i want them.

tldr: can i get my scene window(s) to be 800x600 resolution? (24" monitor 1920x1080 resolution)

http://img295.imageshack.us/img295/1958/goofyscreen.png

Not a full answer but:

Firstly, if you pull the Game Tab off into its own window you can then see what your camera is seeing all the time. Thia helps a lot for me when laying out a GUI.

Secondly, If you notice under the Game tab it says "Free Aspect" in your image. In case you didn't know you can click on that area for a drop-down of resolutions so that your Game view is accurate.

Go to Edit > Project Settings > Player. From here change "Default Screen Width" and "Default Screen Height" to 800 x 600, this way your Game view in the editor will be 800 x 600.

Also when creating your GUI if you expose the values for the x and y screen position of the GUI elements in question, then you can visually edit them by changing the values and previewing them in the Game view since it is not obscured by the Scene window (if you pulled the Game tab off as suggested).