Sprite colors not displaying properly after I click play

For some reason, the colors for my Mega Komaru sprite get all weird when I click Play. This is what it’s supposed to look like:

And this is what it looks like after I click play:

How do I fix this? Thank you!

I am not sure, but maybe if you change the background color to black that might help ?

Do you have any lights in the scene? If yes, please remove them to see if it works