I have a prefab that is being instantiated in various locations in the canvas, and I want to check if it is within a particular trigger collider that is positioned above the canvas. I have tried using both OnTriggerEnter2D and OnTriggerStay2D in the script attached to the prefab, but neither of them seem to work as expected. Despite being instantiated within the trigger collider, the OnTrigger methods are not being called.
Sounds like you’re not meeting all the requirements to receive those callbacks, such as having a Rigidbody2D on the object, naming the callback correctly, etc.
Start with the manual documentation. It’s pretty clear what you need.
I also just verified now that Instantiating a Rigidbody2D object already inside a trigger WILL give you both enter and stay callbacks: