Wrong Sprite Format/Background

I tried to add a sprite to unity, but the colors some pixels were mixing and off.
I found the problem, and it was the format of the file in the inspector. I changed it from automatic to RGB 16 Bit, but when I did the background was all black and no longer transparent. I Don’t know how to make the background transparent again.

@Yword Thank you! I think it was just the compression for me Because I couldn’t adjust the other settings with it on sprite mode, but the compression worked perfectly!